Journal: ChemMedChem

Article Title: Covalent Complex of DNA and Bacterial Topoisomerase: Implications in Antibacterial Drug Development

doi: 10.1002/cmdc.201900721

Figure Lengend Snippet: Figure 2. A) SPR sensorgrams obtained in the selective binding test for

Article Snippet: The experimental SPR sensorgrams were measured using a Biacore T200 instrument and were evaluated using the Biacore T200 evaluation software version 1.0 available in the Biacore Molecular Interaction Shared Resource (BMISR) facility at Georgetown University.

Techniques: Binding Assay

Journal: The Journal of Biological Chemistry

Article Title: Mouse mucosal-associated invariant T cell receptor recognition of MR1 presenting the vitamin B metabolite, 5-(2-oxopropylideneamino)-6- d -ribitylaminouracil

doi: 10.1016/j.jbc.2024.107229

Figure Lengend Snippet: Steady-state affinity measurements of mouse MR1-restricted TCRs. The affinity of TCR-MR1-Ag interactions were determined using SPR, by measuring the binding of soluble mouse MAIT TCRs, M2A ( top panels ), M2B ( middle panels ), and human MAIT, A-F7 ( bottom panels ), against mouse MR1 refolded with 5-OP-RU and Ac6-FP, and human MR1 refolded with 5-OP-RU. The K D values represent mean ± s.e.m values obtained from two independent experiments (n = 2) performed in technical duplicates using different batches of proteins. NB, no binding. RU, response units. The SPR sensorgrams, equilibrium curves and steady state K D values (μM) were prepared in GraphPad Prism 10. 5-OP-RU, 5-(2-oxopropylideneamino)-6-D-ribitylaminouracil; Ac-6-FP, acetyl-6-formylpterin; MAIT, mucosal-associated invariant T; SPR, surface plasmon resonance; TCR, T-cell receptor.

Article Snippet: The SPR sensorgrams, equilibrium curves and steady state K D values (μM) were prepared in GraphPad Prism 10.

Techniques: Binding Assay, SPR Assay

Journal: Journal of Chemical Information and Modeling

Article Title: Active Learning-Guided Hit Optimization for the Leucine-Rich Repeat Kinase 2 WDR Domain Based on In Silico Ligand-Binding Affinities

doi: 10.1021/acs.jcim.5c00588

Figure Lengend Snippet: Experimentally measured binding properties of hit molecules. SPR sensorgrams, fragments (see Figure S2 for full spectra) of NMR spectra of fluorinated compounds (10 μM compound with 0 [black], 20 [red] μM protein), and chemical structures are shown. Solubility and aggregation of compounds as measured by DLS, as well as the set from which each compound was identified, are indicated.

Article Snippet: Eleven hit candidates were advanced to dose–response experiments, eight of which had measurable dissociation constant K D better than 250 μM and acceptable SPR sensorgrams (Chi 2 < 10% R max ; T ( K D ) > 1), with K D values ranging from 18 to 230 μM ( and Table S1 ).

Techniques: Binding Assay, Solubility